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Statistics and reproducibility Statistical significance was determined using independent t -test for immunohistochemical and western blot analyses, and one-way analysis of variance for cell cycle and apoptosis detection by GraphPad Prism 7.0 software (GraphPad, Inc., La Jolla, CA, USA)

In the AMPLITUDE-O post-hoc analysis, the HR for MACE was 0.70 (95% CI 0.371.30) with baseline SGLT2is and 0.74 (95% CI 0.580.94) without SGLT2is ( P -value for interaction 0.70), and the HR for HHF was 0.23 (95% CI 0.050.97) with SGLT2is and 0.70 (95% CI 0.421.17) without SGLT2is ( P -value for interaction 0.35) [47]

This active model predicts and coherently explains all data examined, which included stress, sleep, caloric restriction, stroke, type 1 diabetes mellitus, obesity, and type 2 diabetes

B12 has a very low toxicity, so large amounts are not considered dangerous